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KMID : 0869520080140020074
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Journal of East-West Nirsing Research
2008 Volume.14 No. 2 p.74 ~ p.80
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Screening of Inhibitory Effects of an Oriental Herb on Melanogenesis
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Kang Kyoung-Ah
Han Sang-Sook
Lee Mu-Hyoung
Kim Youn-Jung
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Abstract
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Purpose: To screen candidate oriental herb material for antimelanogenics.
Methods: Oriental herbs (n=100) were screened for mushroom tyrosinase inhibitory activity in vitro using the HM3KO human melanin cell line cultured in DMEM supplemented with 10% fetal bovine serum. Cytotoxicity was assessed by a cell viability assay involving 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Trypan Blue exclusion, and cell enumeration.
Results: Tyrosinase inhibitory effects on 100 oriental herbs was evident. Of these, 11 herbs inhibited tyrosinase activity by 40% without being cytotoxic to HM3KO cells. Three herb varieties significantly decreased melanin synthesis in HM3KO cells.
Conclusions: Oriental herb can have antimelanogenic effects indicating their potential for functional therapeutic use in dermatological whitening.
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KEYWORD
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Melanin, Tyrosinase, Whitening, Herb
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